There are two different kinds of groups you can create to help organize your references: groups and smart groups.
Groups
Here are instructions for creating a simple group in EndNote.
From the top toolbar, select Groups > Create Group
The new group folder will appear in the left column.
Name the group folder.
You can now drag and drop references into the group.
To delete a reference from the group, right click on the reference from the group list and select the option to remove from the group. Do not drag the reference to the EndNote trash because that will delete the reference from your overall library of references (All References folder).
NOTE: References added to any group folder will also remain under the All References folder in your library.
Smart Groups
Smart groups will automatically sort references for you depending on the criteria you set up. For example, you can tell EndNote to send references to a group that contains a specific keyword in the reference.
From the top toolbar, select Groups > Create Smart Group
In the smart group pop-up box:
Name the smart group.
Define what you want EndNote to include in this group. Set up criteria for authors, journal title, words in an abstract, or words that appear in any field of a reference.
Click Create and your references will sort automatically, including any new references that you add later.
NOTE: References added to any group folder will also remain under the All References folder in your library.